1. Brief

The BIOKAU^® Porcine reprodutive and respiratory syndrome virus antibody test kit(PRRSV Ab) is used for the detection of PRRSV antibodies in swine serum; assessment of immunity conditions against porcine reprodutive and respiratory syndrome virus in the pig farms; and investigation epidemiology of the PRRSV.

2. Principle

The BIOKAU^® PRRSV Ab ELISA test kit is made from the antigen coated microtiter plate, goat-anti-pig IgG-HRP and other reagents. It applies the indirect ELISA principle to test the antibodies against PRRSV in porcine serum. In the test, the coated antigen combine with PRRSV-IgG in serum, then add IgG-HRP to specifically bind with complex of antibody-antigens on the microplate. With the TMB substrate, it will generate an amount of color. The depth of color is relative with the content of the PRRSV-IgG.

3. Components

1          PRRSV Coated Microplate.............................................. 2 plates (96 wells)

2          PRRSV Negative control (green lid).................................... 1 tube (1.5 mL)

3          PRRSV Positive control (red lid)........................................ 1 tube (1.5 mL)

4          PRRSV Enzyme Conjugate (yellow lid)................................ 1 bottle (22 mL)

5          Wash Concentrate (20×) (white lid)..................................... 1 bottle (50 mL)

6          Substrate A (purple lid)/B (yellow lid)................. 1 bottle each (12 mL/bottle)

7          Stop solution (blue lid)....................................................... 1 bottle (12 mL)

8          Sample diluent solution (transparent lid).............................. 1 bottle (25 mL)

9  Adhesive Foil................................................................................. 6 pieces

10  Instruction...................................................................................... 1 piece

4. Material Required not Provided

1          Microplate Reader(wave length: 450/630 nm).

2          Microplate Washer.

3          Micropipettes, adjustable.

4          Constant temperature box or incubator.

5          Oscillator.

5. Sample requirement

1          The samples are porcine serum, which should be collected with no bacteria. The storage time should be less than 1 week at 2-8 ℃, if for long term, it should be kept at -20℃.

2          Avoid to use the samples with severe hemolysis, precipitate, contaminated by bacteria or protein suspension.

3          The EDTA, heparin sodiun and other anticoagulants will not affect the results.