1. Principle

The BIOKAU^® Neomycin ELISA test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Neomycin residues in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Neomycin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Neomycin in it. This value is compared to the standard curve and the Neomycin concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.5 ppb

Detection limit

Honey............................................................................................. 10 ppb

Chicken/liver, pork/liver, fish, shrimp.................................................. 10 ppb

 Recovery rate

Chicken, pork, honey, fish, shrimp................................................... 85±15%

Liver(chicken or porcine)................................................................. 70±10%

Cross-reaction rate

Neomycin.......................................................................................... 100%

Streptomycin................................................................................... < 0.1%

Gentamicin...................................................................................... < 0.1%

Dihydrostreptomycin........................................................................ < 0.1%

Kanamycin...................................................................................... < 0.1%

Tobramycin...................................................................................... < 0.1%

Sisomicin........................................................................................ < 0.1%

3. Components

1）   Micro-well strips: 12 strips with 8 removable wells each

2）   6× standard solution (1 mL each): 0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb and 40.5 ppb

3）   Enzyme conjugate (7 mL)............................................................... red cap

4）   Antibody concentrated solution (7 mL).......................................... blue cap

5）   Substrate A solution (7 mL).......................................................... white cap

6）   Substrate B solution (7 mL)......................................................... black cap

7）   Stop solution (7 mL).................................................................. yellow cap

8）   20× concentrated washing buffer (40 mL)..................................... white cap

9）   2× concentrated redissolving solution (50 mL)..................... transparent cap