1. Principle
The BIOKAU® Neomycin ELISA test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Neomycin residues in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Neomycin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Neomycin in it. This value is compared to the standard curve and the Neomycin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.5 ppb
Detection limit
Honey............................................................................................. 10 ppb
Chicken/liver, pork/liver, fish, shrimp.................................................. 10 ppb
Recovery rate
Chicken, pork, honey, fish, shrimp................................................... 85±15%
Liver(chicken or porcine)................................................................. 70±10%
Cross-reaction rate
Neomycin.......................................................................................... 100%
Streptomycin................................................................................... < 0.1%
Gentamicin...................................................................................... < 0.1%
Dihydrostreptomycin........................................................................ < 0.1%
Kanamycin...................................................................................... < 0.1%
Tobramycin...................................................................................... < 0.1%
Sisomicin........................................................................................ < 0.1%
3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb and 40.5 ppb
3) Enzyme conjugate (7 mL)............................................................... red cap
4) Antibody concentrated solution (7 mL).......................................... blue cap
5) Substrate A solution (7 mL).......................................................... white cap
6) Substrate B solution (7 mL)......................................................... black cap
7) Stop solution (7 mL).................................................................. yellow cap
8) 20× concentrated washing buffer (40 mL)..................................... white cap
9) 2× concentrated redissolving solution (50 mL)..................... transparent cap