1. Brief

The BIOKAU^® hog cholera virus antibody test kit, or called Classical Swine Fever Virus Antibody ELISA Test Kit (CSFV Ab), is used for the detection of hog cholera virus antibodies in porcine serum; assessment of immunity conditions against hog cholera virus in the pig farms and epidemiology investigation of the hog cholera.

2. Principle

The GreenSpring^® hog cholera virus antibody ELISA test kit is made from the antigen coated microtiter plate, goat-anti-pig IgG-HRP and other reagents. It applies the indirect ELISA principle to test the antibodies against hog cholera virus in porcine serum. In the test, the coated antigen combine with CSFV-IgG in serum, then add IgG-HRP to specifically bind with complex of antibody-antigens on the microplate. With the TMB substrate, it will generate an amount of color. The depth of color is relative with the content of the CSFV-IgG, when the value of color is greater than the cut-off value, the pigs are vaccinated well.

3. Components

1  CSFV coated microplate.................................................. 2 pieces (96 wells)

2  CSFV Negative serum(green lid).............................................. 1 tube (1 mL)

3  CSFV Positive serum (red lid).................................................. 1 tube (1 mL)

4  CSFV Enzyme conjugate (yellow lid).................................... 1 bottle (22 mL)

5  20×Wash Concentrate (white lid)........................................... 1 bottle (50 mL)

6  Substrate A (red lid)/B (black lid)......................... 1 bottle each (12 mL/bottle)

7  Stop solution(blue lid).......................................................... 1 bottle (12 mL)

8  Sample diluent solution(transparent lid)................................ 1 bottle (25 mL)

9  Adhesive Foil................................................................................. 6 pieces

10  Instruction...................................................................................... 1 piece

4. Material Required But not Provided

1          Microplate Reader(wave length: 450/630 nm).

2          Microplate Washer.

3          Micropipettes, adjustable.

4          Constant temperature box or incubator.

5          Oscillator.

5. Sample requirement

1          The samples are porcine serum, which should be collected with no bacteria. The storage time should be less than 1 week at 2-8 ℃, if for long term, it should be kept at -20℃.

2          Avoid to use the samples with severe hemolysis, precipitate, contaminated by bacteria or protein suspension.

3          The EDTA, heparin sodiun and other anticoagulants will not affect the results.