1. Brief
This product is used for testing Total Aflatoxins residue in maize sample. The total test time only
need 5~10 min. Easy to operate, high sensitivity.
2. Detection limit
3. Specifications: 50 tests/kit
4. Principle
The Total Aflatoxins rapid test strip is an immuno-chromatographic test, using colloidal gold
immunoassay method. It relies on the competition between Total Aflatoxins residues in the sample
and? the Total Aflatoxins on T line, to the limited amount of dye-antibody. As a sufficient amount of
drug in the sample is presenting, the drug will saturate the antibody. Consequently, it will show an
extremely light or even invisible T line, indicating a positive result. On the other hand, if there is a
negative sample (or the amount of the drug is lower than the minimum detectable concentration), it
will generate two obvious lines in both the T and C line section.
5. Contents
1) Test card 50 pieces
2) Manual 1 piece
3) Dropper 50 pieces
4) Desiccant 1 piece/bag
5) Disposable gloves 3 pieces
6) 2ml Centrifuge tube 50 pieces
7) Micro-well 50 pieces
8) 0.01M PBS buffer 6ml
6. Sample preparation
Maize
Weigh 0.5g maize flour (maize is crushed) into 2ml centrifuge tube, add 1ml methanol, shake
vigorously by hand or on vortex for 1min, be static for 5min until obvious stratification, use dropper
to absorb 4 drops of clear up-layer liquid (or use micropipette to take 100ul) into micro-well, blow to
dry in boiling water or by hair drier, then add 80ul 0.01M PBS buffer to dissolve it. Use dropper blow
and beat repeatedly for 10s. Use dropper transfer all liquid to test well.