1. Principle

This kit is based on the specific reaction of antibody-antigen and immunochromatography. The Aflatoxin M1 in the sample compete for the antibody with the antigen coated on the membrane of the test strip. Then after a color reaction, the result can be observed.

2. Applications

This kit is used for rapid qualitative analysis of Aflatoxin M1 in milk sample.

3. Detection Limit (LOD)

50 or 500ppt

4. Kit components

(1) 8microwellsand 8 test strips in one bottle. 12 bottles / kit.

(2) Instruction

5. Operations

(1) Read the instructions carefully before experiment. Bring the test kit and samples to room temperature. Milk samples should be fully liquid without any agglomeration and deposition.

(2) Take bottles needed from the kit package, take out required wells and strips, make proper marks. Please use these test strips within 1h. Seal the cap of the bottles.

(3) Take 200ul of the test samples into the wells, then repeatedly absorb for 5 times, mix the sample with the reagent in the wells completely. The mixture should be pink, and then start the timer.

(4) Incubate for 5min at room temperature (25℃), insert the test strips into the wells with the "MAX" end fully dipped in to the mixture.

(5) Incubate for 5min at room temperature again. Take out the strip; judge the result according to Part 6.

6. Result

Negative(-) : Line T and Line C are both red,and Line C has less than Line T;

Positive(+) : Line C is red, line T has no color(Aflatoxin M1＞500ppt) or Line C has no less than Line T (Aflatoxin M1＞50ppt);

Invalid: Line C has no color, which indicates the strips are invalid. In this case, please read the instructions again, and redo the assay with new strip.

Note: If the result of the strip needs to be recorded, please cut the foam cushion of the "MAX" end, and dry the strip, then keep it as file.

7. Specificity

The kit has no cross reactions with clenbuterol, ractopamine, salbutamol, melamine, trenbolone, sulfonamides, chloramphenicol, macrolides and other drugs.

8. Storage

2-8℃ in cool dark place, do not freeze. The kit will be valid in 12 months. The lot number and expired date are printed on the package.

9. Notice for operations

a)    Please do the assay following the instruction, don not touch the membrane of the strip.

b)    Please seal the bottle after taking out required strips.

c)    This strip is used for only once; please do not use it repeatedly.

d)    This kit is only for screening test, positive result should be further confirmed with other method.