1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Chloramphenicol antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chloramphenicol in it. This value is compared to the standard curve and the Chloramphenicol concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.05 ppb

Detection limit:

Intestine......................................................................................... 0.1 ppb

Honey.......................................................................................... 0.15 ppb

Milk.............................................................................................. 0.05 ppb

Urine, serum................................................................................... 0.1 ppb

Chicken/liver, pork/liver, fish, shrimp............................................... 0.05 ppb

 Recovery rate

Chicken/liver, pork/liver, fish, shrimp................................................ 80±10%

Honey............................................................................................ 70±10%

Milk................................................................................................ 85±15%

Cross-reaction rate:

Chloramphenicol................................................................................ 100%

Thiamphenicol................................................................................. < 0.1%

Florfeniol......................................................................................... < 0.1%

3. Components

1）   Micro-well strips: 12 strips with 8 removable wells each

2）   6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb

3）   Enzyme conjugate (12 mL)............................................................. red cap

4）   Concentrated antibody working solution (1 mL).............................. blue cap

5）   Substrate A solution (7 mL).......................................................... white cap

6）   Substrate B solution (7 mL)......................................................... black cap

7）   Stop solution (7 mL).................................................................. yellow cap

8）   20× concentrated washing buffer (40 mL)..................................... white cap

9）   2× concentrated redissolving solution (50 mL)..................... transparent cap